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济南大学化学系,山东,济南,250002
纸质出版日期:1999,
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[1]王蔚.纤维素酶合成机制的探讨[J].济南大学学报,1999,{4}(05):63-65.
WANG Wei. STUDY ON MECHANISM OF CELLULASE SYNTHESIS[J]. Journal of University of Jinan(Social Science Edition), 1999,(5):63-65.
[1]王蔚.纤维素酶合成机制的探讨[J].济南大学学报,1999,{4}(05):63-65. DOI:
WANG Wei. STUDY ON MECHANISM OF CELLULASE SYNTHESIS[J]. Journal of University of Jinan(Social Science Edition), 1999,(5):63-65. DOI:
纤维素酶是一种诱导酶,纤维素降解物葡萄糖对纤维素酶的合成具有阻遏作用;山梨糖可作为一种诱导剂来提高纤维素酶的酶活。本实验采用向Mandel无机盐营养液中加入不同浓度的山梨糖来观测山梨糖的诱导作用;另外,将经Co60 照射后的孢子悬液倾注于含不同浓度葡萄糖的纤维素双层平板上,来筛选抗葡萄糖阻遏的菌株。
Cellulase is an inducible enzyme which can be induced by some oligomeric sugars and their derivatives such as lactose
cellobiose and sophorose. According to the research
its synthesis is regulated by induction repression mechanism. In this experiment
we use sophorose as an inducer to improve enzyme activity; Because glucose can repress the expression of cellulase
we can screen glucose resistant strain on the plate which contains glucose.
绿色木酶纤维素酶山梨糖葡萄糖
Trichoderma reeseiCellulaseSophoroseGlucose
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